C (3-6 months) Developmental Biology Research true Cancer Research B6-Sparc KO B6-Sparc KO 奥羽大学歯学部・加藤靖正先生。C57BL/6コンジェニック。The Wistar Institute・Chin Chen Howeにより開発。CCB ES細胞を用いて作出。Benaroya Research Institute at Virginia Maon・Helene Sageより分与（2001）されたものより育成。 Kato, Yasumasa 加藤 靖正 C（3〜6か月） Developed by Chin Chen Howem The Wistar Institute. CCB ES cells were used to generate the knockout mice. The mutant mice were maintained by Yasumasa Kato, Ohu university School of Dentistry through Helene Sga, Benaroya Research Institute at Virginia. C57BL/6 congenic. Hematological Research In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Invest. Ophthalmol. Vis. Sci., 39, 2674-2680 (1998 ). RBRC06313 Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygote x Homozygote [or Crossing to C57BL/6JJcl] 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Invest. Ophthalmol. Vis. Sci., 39, 2674-2680 (1998 ). CCE/EK.CCE [129S/SvEv-Gpi1<c>] Cell Biology Research polyoma virus thymidine kinase gene promoter, E. coli neomycine resistance gene, mouse RNA polymerase II promoter, bovine growth hormone gene polyadenylation signal, mouse Sparc genomic DNA Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> B6.129-Sparc<tm1Hwe> B6.129-Sparc<tm1Hwe> Sparc (osteonectin, BM-40) gene knockout mice. Exon 4 of Sparc gene was replaced with MC1-neo and Pol2-neo tandem cassettes. Homozygous mutant mice exhibit cataracts, abnormal skeletal morphology and osteopenia (viable and fertile). <a href='https://brc.riken.jp/mus/pcr06313'>Genotyping protocol -PCR-</a> Sparc (secreted acidic cysteine rich glycoprotein) 遺伝子のノックアウトマウス。エクソン4をMC1-neo/Pol2-neoカセットで置換。ホモマウスは白内障、尻尾の変形、osteopeniaを呈する。繁殖は正常。SPARCは、osteonectin、BM-40としても知られるマトリセルラー蛋白である。骨における非コラーゲン性タンパク質としても最も多く存在している。細胞接着や増殖、血管新生などに影響する。種々の癌で高発現が認められている。